Tell you about the virus preservation solution
Virus preservation solution is also called cell preservation solution, and both are the same thing.
In the conventional sense, the virus preservation solution can also be used to freeze human and seeded animal cell lines. The specific function is for cell culture, introduction, preservation, and important technical means to ensure the smooth progress of experiments.
What are the advantages of virus preservation solution?
In today's era, glycerol or dimethyl sulfide is often used as a protective agent for the cryopreservation of cells or viruses. These two components can increase the permeability of the cell membrane to water, and slow freezing technology can make the water contained in the cells Exudation reduces the probability of ice crystal formation in cells, thereby reducing cell damage caused by ice crystal formation. If the cells need to be resuscitated, it is recommended to use a rapid melting method. This will ensure that the extracellular crystals will melt in a short period of time, and avoid damage to the cells due to the slow melting and the formation of intracellular recrystallization. Non-program cooling. It can be stored in a low-temperature refrigerator at -80℃ for up to 5 years, which can ensure the safety of cryopreserved cells. The perforated plate (cell culture plate) can be used for cryopreservation of hybridoma cells.
The virus preservation solution has a dilution function, which can separate a large number of effective cells embedded in the mucus, so that more cells with experimental value can be properly preserved, providing a sufficient total number of cells, and ensuring the accuracy of the test results. At the same time, the processed sample can be completely removed from the sample after being filtered by low-speed centrifugation, effectively preventing the interference of the sample from the sample test result. After low-speed centrifugation, the virus preservation solution can be made into a uniform monolayer cell sheet, which meets the inspection requirements.
Scan QR code and WeChat consultation